Microbial growth and adhesion of Escherichia coli in elastomeric silicone foams with commonly used additives

Silicone is often used in environments where water repellency is an advantage. Contact with water promotes the adhesion of microorganisms and biofilm formation. Depending on the application, this may increase the possibility of food poisoning and infections, the material's degrading appearance, and the likelihood of manufacturing defects. The prevention of microbial adhesion and biofilm formation is also essential for silicone-based elastomeric foams, which are used in direct contact with human bodies but are often difficult to clean. In this study, the microbial attachment in and the retention from the pores of silicone foams of different compositions is described and compared to those of commonly used polyurethane foams. The growth of the gram-negative Escherichia coli in the pores and their leaching during wash cycles is characterised by bacterial growth/inhibition, adhesion assay, and SEM imaging. The structural and surface properties of the materials are compared. Despite using common antibacterial additives, we have found that non-soluble particles stay isolated in the silicone elastomer layer, thus affecting surface microroughness. Water-soluble tannic acid dissolves into the medium and seems to aid in inhibiting planktonic bacterial growth, with a clear indication of the availability of tannic acid on the surfaces of SIFs.

Silicone is a well-researched material with a broad range of applications. Nevertheless, its antibacterial properties in specific applications still raise questions. In medical applications, silicone-based foams (SIFs) are primarily used as prostheses 1 and modern wound dressings 2,3 , constantly contacting body tissue and body fluids. As environmental humidity and water promote biofilm formation by the adhesion of microorganisms, such conditions undoubtedly increase the possibility of infections. Another rapidly growing field of application for SIFs is cushioning (seats, mattresses, gaskets), where occasional contact with body fluids, food, and liquids is highly probable. Cushioning foams often have an open-celled structure, which allows the penetration of air, fluids and microorganisms. As biofilm formation also degrades the appearance of the material and increases the likelihood of manufacturing defects 4,5 , the prevention of microbial adhesion and biofilm formation on silicone materials is an important subject, independent of their application.
Poly(dimethylsiloxane), e.g. PDMS-based polymers, commonly known as silicones, are not inherently antibacterial. Additives such as catalysts, including platinum nanoparticles 6 , and other low molecular weight species incorporated between polymer chains or grafted onto the polymer backbone may give silicone antibacterial activity [7][8][9] . Low surface tension and, therefore, high hydrophobicity is reported to be one of the primary reasons PDMS is prone to protein adsorption and bacterial adhesion 10,11 . For example, Busscher et al. compared Candida albicans and C. tropicalis and found that the more hydrophobic the surface of the microorganism, the more it is inclined to adhere to a silicone surface 4 . Although the gram-negative bacteria Escherichia coli have both hydrophobic and hydrophilic regions in its outer membrane layer, its surface is generally considered hydrophilic (the contact angle for wetting is reported to be in the range of 16.7°-24.7°) 12,13 . It is generally understood that the adhesion of microorganisms depends on hydrophobic interactions between the bacterial cell and the polymer surface 13 .
Seeking to suppress the adhesion of hydrophilic bacteria onto a hydrophobic surface, increasing surface hydrophilicity 10,14,15 is often proposed as a possible solution. The adhesion of E. coli onto silicone catheter is shown to decrease by 32% by grafting antimicrobial peptide and polyvinylpyrrolidone on cured PDMS, or even up to ~ 95% by using vinyl-modified methyl cellulose 16 and, using carboxymethyl chitosan and polydopamine resulted in a ≥ 90% reduction in E. coli adhesion 15 . Also, grafting acrylates 7 to silicone rubber (Pseudomonas, catheter) effectively suppresses non-specific protein adsorption and cell adhesion, suppressing hydrophobic recovery of the surface. One of the most recent works by McVerry et al. shows a successful one-step hydrophilic surface modification in ambient conditions and under UV light to create a zwitterionic polymer polysulfobetaine and perfluorophenylazide network onto silicone surface 17 . The reported antibacterial activity was due to the formation of the surface hydration layer in the presence of the hydrophilic coating.
The surface modifications mentioned above are successfully conducted for monolithic silicone, allowing it to process the material's surface uniformly. However, it becomes much more challenging to graft the highly porous and low-density open-celled SIFs throughout the structure. Submerging large air-filled volumes would be cumbersome and time-consuming. Also, when moulding, a partial skin forms during the manufacturing process. Most importantly, the hydrophobic nature is instead favoured to deter water from its surface and pores, especially in cushioning and insulation applications. Functionalising its surface with hydrophilic groups would revoke its water-deterring property, thus acting as a sponge-like material.
To maintain the material's hydrophobic nature, it is possible to add local antibacterial sites in the form of particles, thus inhibiting the growth of microorganisms. These particles may partially surface during the thinning of pore walls in the curing and foam-formation process but remain embedded in the material. Such a fabrication method is based on the polymerisation and dispersion of components and avoids the wearing of antibacterial additives.
To apply antibacterial properties to silicone, incorporating different silver species (nanoparticles, salts, ions) has been one of the leading research directions for many years [18][19][20][21] . In conjunction with hydrophobic silicone, wound dressings are found to benefit in extracting bacteria from wounds and act bactericidal simultaneously 2 . Inorganic nanoparticles, Ag, ZnO, and TiO 2 show high antibacterial efficacy against E. coli, but their use in effective concentrations alters mechanical properties significantly 20 . For large volumes of material, several lowcost additives and fillers could be incorporated into the foam matrix. For example, tannic acid [22][23][24] , shungite 25 , chitosan 26 , mica, and zinc-based 27 have shown antibacterial activity.
The microbial activity on silicone foams has been investigated mainly for commercial wound dressings 2,19 through qualitative (i.e. zone inhibition) and quantitative (bacterial viability assay) tests. In a powdered form, a shake-flask method has been used to test the antibacterial activity of peroxide-cured high-density SIFs, where the antimicrobial effect lies on toxic additives leaking from the (post-)cured foam 28 . For different wound dressings, zone inhibition test results have been compared with the hydrophobicity of the porous material surface 2 . For unfoamed silicone elastomers, an untreated porous membrane has also been shown not to reveal any bacteriostatic or bactericidal activity 29 .
Recently, a standardised method, ISO 23641:2021 30 was published for testing flexible cellular polymers based on the shake flask method and could be used as a guide for evaluating the antibacterial effectiveness. In some cases, a simplified process could be applied as effectively.
This study focuses on the antimicrobial activity in polysiloxane foams with different mineral and organic additives, which are readily available and thus industrially feasible. For comparison, polyurethane-based (PU-, or PUR-) foams and their antibacterial activities are assessed, as PU is the most widely used material in the previously mentioned applications and many more. There is extensive research on PU-foam and its antimicrobial additives (in wound dressings 19 , membranes 31 , composites 31 and coatings 31 ), but for SIFs, many research questions are still unanswered as this field is constantly evolving. More importantly, both materials are applied as cushioning layers in seats and mattresses. As elastomeric silicone foams are increasingly gaining interest in cushioning, vibration dampening, insulating 32 and medical applications, tuning their antimicrobial properties add value to their already superb nature.

Results and discussion
Antibacterial effect on the growth medium. This experiment shows how the different additives in the foam and the difference in base material affect bacterial growth in the inoculation suspension within 24 h at 25 °C. The resulting concentrations of E. coli in Luria-Bertani medium (LB broth) surrounding the test cubes are depicted in Fig. 1. As the foam cube is immersed in the medium, it is freely permeable to the bacteria and the carrying medium. E. coli concentration is expected to differ from the pure, cube-less growth medium and around cubes proposing an antibacterial effect. For this, the same tests were conducted for a pure inoculation mix (No foam) and a standard silicone foam without antibacterial additives (SIF) for the control experiment.
The results show that the concentration of E. coli (c[CFU]) in the growth medium was the highest around SIFs with activated carbon additive (SIF-AC). Compared to the pristine (additive-free) SIF, the E. coli concentration (CFU/ml) in the surrounding growth medium of SIF-AC is threefold higher. This significant difference in c [CFU] suggests that the hydrophilic activated carbon additive increases the concentration of the growth medium around the foam cubes. Activated carbon is described as an adsorbent to bind molecules from liquids by van der Waals forces, causing a higher concentration of adsorbate at the interface than in the bulk fluid 33 . Therefore, the effect on c[CFU] in growth media around SIF-AC could result from the increased attachment of bacteria on the foam's pore surface, which is also seen on the SEM (Scanning Electron Microscope) images (section Bacterial population formations). Although methyl cellulose's hydrophilic and water-soluble nature would allow it to diffuse from the polymer composite and dissolve in LB, its inclusion in the SIF matrix (in SIF-MeC) does not have a significant effect on inhibiting bacterial growth in the medium compared to pristine SIF. A foam-less growth medium resulted in 1/3 logs lower c[CFU] than with the SIF foam specimen, which suggests that the presence of a SIF foam cube increases the E. coli /LB concentration in 24 h at 25 °C. www.nature.com/scientificreports/ The inoculation suspensions around SIF-SHU, with shungite additive, resulted in lower c[CFU]-s than for pristine SIF, although it is essential to notice that the standard deviation ranges overlap; hence the difference may not be significant. As shungite is reported to have antibacterial properties in an aqueous medium, we expected an effect on c[CFU] 25 . SIFs that significantly hinder the growth of E. coli at 25 °C are SIF-CHI and SIF-TAN. Compared to pristine SIFs, the hydrophilic chitosan additive in SIF-CHI seems to have a distinguishable effect in inhibiting bacterial growth in the medium. As chitosan is water-insoluble, its dissolution from the polymer is not expected. Its protrusion from the surface to some degree or availability from the cut sides of the foam is possible. From previous research by Qin et al., the water-insoluble chitosan shows an inhibitory effect against E. coli due to the water acting as an acidic medium 34 . For SIF-TAN, during the inoculation period, a visually detected discolouration of the growth medium indicates tannic acid (TA) leakage into the medium. The leakage is further promoted by cutting the sample and exposing TA to the solution. As TA is a highly soluble molecular substance, vibrational diffusion of the molecules from a hydrophobic cross-linked polymer network and dissolution into an aqueous medium is possible 35 . The SIF-TAN foam sample resulted in the best antibacterial effect among other additive-doped foams in this test, resulting in 0.5 logs lower c[CFU] than for pristine SIF.
In general, cell adhesion to hydrophobic surfaces has been previously described by McVerry et al. as due to the nonpolar nature of PDMS and the significant increase in antibacterial effect when its surface is modified to hydrophilic 17 . As the surface of PUR is naturally hydrophobic 36 but less hydrophobic than silicone, bacterial adhesion and growth in the surrounding medium are expected when there is a possibility to use the material's surface for attachment and multiplication. Contact angle measurements (see Supplementary data S1) show that although both SIF and PUR are hydrophobic (Θ > 90°), the water droplet wets PUR and PUR-EG/APP surface more efficiently than SIF and SIF-AC surfaces. We have observed the wetting to increase in time due to the porous nature of the foams.
Despite a 0.5 log difference in c[CFU] between SIFs and PURs, we also need to consider the structure of the foam. Near similar foam densities, SEM micrographs reveal comparably larger voids in the PURs, which suggests that the bacteria have less surface area to adhere to and, therefore, to multiply. Also, the vigorous movement of the medium during shaking could hinder the adhesion of bacteria. The corresponding images comparing foam structures can be found in the Online Appendix S1 of this manuscript. E. coli adhesion, growth, and leaching from porous foam. Samples, which were inoculated in E.coli/LB for 24 h at 25 °C (180 rpm), were washed in 1 × PBS for five sequential cycles to assess the adhesiondetachment behaviour of bacterial cells from the pores (planktonic cells) and pore walls (adhered cells). The following results in Fig. 2A,B indicate how different materials affect bacterial adhesion and growth and whether the antibacterial additive has any effect inside the foam. From the first wash (wash I), the c I [CFU] leaching is in correlation with the c 24h [CFU] being highest for polyurethanes and lowest for SIF-TAN/SIF-CHI. Comparing the c[CFU] of wash mediums shows that a relatively large portion of bacteria leaches out with the 1st wash indicating the majority to be planktonic, e.g. floating inside the foam. The sum of bacteria washed out from the foam would not give us the overall bacteria inside the material, as not all cells are released within five washes. Still, the subsequent washes (from wash II-V) reveal more bacteria present in total than in the surrounding medium.
A steeper slope at the beginning of Fig. 2 depicts the extraction of loose (planktonic) viable cells. We suggest that the shallow plateau at the end of the curves (washes III, IV, and V) could indicate the slow detachment of adhered cells from the pores (Fig. 2A). The steepest curves from washes I-III project well the highly porous structure of PUR and PUR-EG/APP, allowing faster passage for the suspension. Contrary to PURs, the bacteria  www.nature.com/scientificreports/ are more difficult to extract from the SIFs. Possible mechanisms include the more probable adhesion to the more hydrophobic surfaces and the obstruction of the smaller pore size, which lowers the rate of liquid exchange during washes. As the SIF-TAN had the lowest E. coli concentration in the surrounding growth medium after 24 h of incubation, the early plateauing curve is expected due to the initially fewer bacteria present and the antibacterial effect due to the leaking of TA into the solution.
We were also interested in whether there was significant bacterial growth in the foam cube or whether the results from washes were merely the effect of adhesion. One possibility was to compare if the attachment/ adhesion of E. coli is affected by the inoculation duration. The differences between short-term (0 h, immediate washout) and long-term (24 h) inoculations were analysed, and the summary is depicted in Fig. 3A.
As before, the steeper curve allows us to assume that more bacteria are loose (planktonic) and less adhered. As expected, a more extended period has allowed the bacteria to grow but also to interact with the surface of the foam material. The wash curves after 24-h incubation period show slower elimination from the pores than for immediately washed out bacteria. Such plateauing could indicate gradual detachment behaviour from the hydrophobic material.
To illustrate the growth effect in low humidity conditions, as well as in lack of nutrients, we conducted a test in a closed environment at 25 °C without additional broth or shaking, followed by five subsequent washes (AIR test, Fig. 3B). Compared to the previously used method in this research, which is incubation in a flask and  www.nature.com/scientificreports/ LB medium in excess, the elimination of bacteria from the foam samples results in somewhat similar curves of washout concentrations, indicating that when the bacteria have sufficient time to grow and multiply, there is enough time to adhere to the material's surface.
Structure and surface of foams. All silicone foams used in this study are open-cell elastomeric polysiloxane-based foams. The foams were prepared using non-antibacterial general fillers distributed into the prepolymers. Such filler particles are visible under the polymer layer (see Fig. 4A) of a pristine SIF. The surface with antibacterial additives differs only slightly from the pristine SIF visually by surface roughness and particle distribution, as seen in the middle image (Fig. 4B, SIF-MeC). Although general fillers used in SIFs compositions were not used for the standard PUR and PUR-EG/APP, the EG or APP particles used in this composition were not visibly detected protruding the surface (Fig. 4C). Antibacterial-purposed additives make up a relatively large portion of the pre-mixture, varying from 0.3 to 5.0 wt%. The spatial dimensions of the particles compared with the pore wall thickness should allow them to surface/protrude during the blowing process and the consequent wall thinning. Therefore, we expect them to affect bacterial growth upon partial or direct contact with bacteria when the additive can disturb the cell membrane causing cell lysis and death 15 .
However, it is possible that due to the synthesis conditions and resulting foam parameters (wall thickness, additive content and particle dimensions, but most importantly, surface tension), these particles do not surface fully, and the majority remain covered with a thin polymer layer. To confirm this phenomenon, we performed EDX analysis on the SIFs surface by focusing on 20 different areas to acquire the surface's elemental composition. The distribution of elements (Si, C) is shown on the elemental maps obtained by the SEM-EDX technique (Fig. 5).
Compositional imaging results suggest that the particles are well incorporated into the silicone pre-polymers, leaving tangled polymer chains on the surface of the additives. For example, carbon-dense shungite areas in the polymer matrix are depicted by obtaining the EDX spectrum. Details of the EDX spectra of the SIFs surface values measured in atomic and weight percentages are listed under images in Fig. 5. Although the elemental composition of additives used is mostly C and H, the SEM-EDX results for SIF-CHI also show a low nitrogen concentration on the pore surface.
Bacterial population formations. Amongst all observed samples, after the 24-h incubation period (without washouts), the bacteria tend to locate in different structural formations on the surface of the SIFs. An even distribution of adhered E. coli is only observed on pristine SIFs and SIF-AC (Fig. 6), which suggests that there is less or no disturbing effect for the bacterial adhesion favoured by the hydrophobic surface characteristic of silicone. The bacteria have also adhered to the sites where fillers are present-without any visible distinguished behaviour on or around it (Fig. 6, upper center image).
Activated carbon with a high surface area is considered hydrophilic (the higher the surface area, the higher the hydrophilicity) and with high adsorption capacity 33 . We found bacteria spread evenly on the pore surfaces in all foams with AC additive and some larger colonies/agglomerations on single sites. The formation of pili, which are mainly responsible for attachment during conjugation, allowing binding to solid surfaces, is evidence of suitable conditions for the gram-negative bacteria to share genetic information and multiply (Fig. 6, bottom center image). The pili were rare in these spread formations but more probable in colony cluster formations.
In addition to similar formations on SIF and SIF-AC, we noticed distinctive behaviour in groups of waterinsoluble additives (shungite, chitosan) and water-soluble additives (tannic acid, methylcellulose). Although shungite has carbon in different modifications and is insoluble, it contributes some dissolving components that pose antibacterial properties 25,37 . SEM analysis reveals that the bacteria are found only in the outmost pores of the SIF-SHU sample leaving the inner pores almost bacteria-free (Fig. 7). Leaking of such components from the skeleton would explain the absence of adhered bacteria in the foam. Initially, the concentration of dissolved particles in the inoculation suspension is lower around the cube where the concentration of E. coli is relatively high (30.5 ml of total inoculation suspension, of that < 2.7 ml in the cube). www.nature.com/scientificreports/ Samples with insoluble chitosan additive (SIF-CHI) show an uneven distribution of adhered E. coli (Fig. 8). We found only a few microorganisms in some pores, and pores closer to the sides of the sample were unevenly populated. Again, some bacteria were as large clusters/agglomerates were present (Fig. 8, image A), which may have been planktonic forms before the supercritical extraction. No such distribution, as was seen for pristine SIF (Fig. 6) and SIF-AC (Fig. 6), was found.
In this research, we have found that the antibacterial effect of methylcellulose (in SIF-MeC) on the surface of the material is similar to chitosan (in SIF-CHI) (Fig. 9B,C), but its antibacterial effect is negligible in the growth medium (Fig. 1). The latter indicates that the methylcellulose and chitosan do affect the adhesion of bacteria in the pores of silicone foam.
Interestingly no bacterial adhesion as described above was visible on pore surfaces in the foam with the tannic acid (TA) additive. During the incubation period with vigorous aeration (180 rpm), highly soluble TA (solubility: 2850 g/L water, 1.7 mol/L) leaked from the foam, detectable from the discolouration of the suspension from yellow to brownish-yellow. As the samples were freeze-cut before inoculation, some TA particles may have become loose and uncovered. In the respective SEM images (SIF-TAN), we did not find any bacteria on the pore walls (Fig. 10). In SIF-TAN, pores are interconnected, and considering the size of the E. coli (2-5 µm), bacteria were able to free float throughout the void space of the foam cube along with the liquid medium. As there is no visible surface colonisation, we assume that the bacteria leaked during washes were floating and multiplying in the inoculation suspension (e.g. staying planktonic). The planktonic lifestyle of microorganisms in similar conditions has also been suggested by Tan et al. 38 .
Compared to the SIFs, the analysed low-density PURs (PUR and PUR-EG) had significantly less surface area to adhere to, and the structure is hollower (Fig. 11, upper row). Interestingly, thread-like bridges from the   www.nature.com/scientificreports/ PU material have formed across pore edges during the foaming and curing process, which is where most E. coli have attached. As the inoculation/growth medium flows through the channels, the planktonic bacteria adhere to these sites, which leads to the assembly of larger colonies. We found no evidence of such activity in SIFs. For PUR-EG foams, fire-retardant polyurethane foams with EG (exfoliating graphite) and APP (ammonium polyphosphate), we noticed that most/all bacteria formed large colonies and almost none on the surface of the cell material (Fig. 11, bottom row). These formations are adjacent to one another. As for PUR, E. coli has also adhered to material bridges of the interconnection pores.
The resulting open-cell foam densities were in the range of 80-175 kg/m 3 and were cut into rectangular pieces of 1.3 × 1.4 × 1.5 cm 3 (volume of 2.7 cm 3 ). The porosity of the foam was calculated from the foam density considering the bulk density of the silicone composite was 1 g/cm 3 . As a reference, two polyurethane foams obtained from the industrial production of Estelaxe OÜ were compared using the same method. The list of test foams can be found in Table 1, which is accompanied by structural information on organic additive molecules in Fig. 12.
Test organism Escherichia coli (E. coli) Nissle strain was used in all experiments. Luria-Bertani nutrient broth (LB) and 0.1 M phosphate buffer saline (1XPBS) were prepared by the Institute of Technology, University of Tartu. Formaldehyde solution (37%) for cell fixation was obtained from Panreac AppliChem. Estelaxe OÜ provided the PU-based foam samples, and polysiloxane-based samples were synthesised in-house.

Methods
Preparation of polysiloxane foams. The prepolymer mixtures were prepared using a stand-alone mixer with a PTFE-covered rotary blade. The components of pre-mixtures were separated into two distinct parts, one accommodating the catalyst and the moderator, the other containing hydride in addition to other functionalised   www.nature.com/scientificreports/ pre-polymers. Both components were mixed separately, then mixed and injected by a lab-designed injectionmoulding device, dispensing a total volume of 500 ml of pre-mixture per foam sample. Obtaining bacteria from growth medium and foam samples. Firstly, aliquots were collected from the 24-h growth medium surrounding the foam cubes. In addition, a series of washes followed to extract the bacteria from the foam sample. Each foam cube was transferred into a sterile Erlenmeyer flask containing 30 ml of 1 × PBS (phosphate buffer saline solution) and followed by a wash cycle of 10 min at 25 °C and 180 rpm. For further analysis, a sample from each washing (PBS and extracted bacteria) was sampled. This step was repeated until 5 washes were conducted.

Determining bacterial concentrations.
We analysed all initial 24-h growth mediums and subsequent wash mediums to determine the bacterial concentrations. OD 600 was assessed by a UV/VIS spectrophotometer (Ultrospec 7000, Biochrom) to evaluate the cell density of the wash samples. The samples were serially diluted tenfold in each step using 1 × PBS. 100 µL of dilutions were plated onto the LB Agar plate and incubated overnight at 37 °C. The bacterial colonies were counted, and initial concentrations were calculated considering specific dilution factors. The antibacterial activity/efficacy can be seen by the decreased ratio of bacteria considering the bacterial concentration of the foamless inoculum. The supercritical CO 2 extraction process, a necessary step for preparing cells for imaging, was conducted using a critical point dryer (E3100, Quorum Technologies) and thermostat (Proline RP 1845, LAUDA). For imaging the cross-sections under SEM (Hitachi TM3000, 15 kV), the samples were freeze-cut using a scalpel and sputter-coated with a 7.5 nm layer of gold.

Visualising the bacterial adhesion in cells via SEM.
Elemental mapping of polymer composite surfaces. SEM-EDX technique was applied to map the elemental distribution and acquire the surface composition of foam materials. For EDX, SwiftED3000 (Oxford Instruments) was utilised in combination with SEM (Hitachi TM3000). Elemental compositions were analysed by collecting data from 20 points, and the results were averaged. www.nature.com/scientificreports/ Contact angle determination. The hydrophilicity of the foam materials was evaluated by measuring the contact angle formed between water droplets and the surface of the polymer foam and the skin-like layer which forms upon moulding. For this purpose, drops of water were mounted on three different areas. Results are the mean value of three measurements on different polymer film parts. Results are summarised in the Supplementary S1.

Conclusions
Antibacterial activity for different silicone foams compared with polyurethane foams was evaluated by inoculating the foams with gram-negative E. coli, one of the most common pathogenic organisms found in mattresses and seat cushions. Different fluids, usually accompanied by microorganisms, are prone to enter the material's pores during the repetitive compression that characterises their application. Therefore, the quantitative method we have applied is suitable for describing the bacterial growth in elastic and three-dimensional structures in conditions where an excess carrying medium is available and the planktonic bacteria are free to adhere to the surface. We focused on comparing the antimicrobial activity of the commercially available and low-cost natural additives integrated into the polymer matrix, as it is somewhat cumbersome to immerse or dip-coat the end product. The following conclusions from the results of this study can be drawn: • While the majority of E. coli's outer membrane is hydrophilic, the combination of partial hydrophobicity and SIF surface microroughness is sufficient to allow the attachment of the bacteria; • The antimicrobial effect, or the lack of it, could be explained by the thin polymer layer covering the additive particles expected to act as antibacterial sites; • Although the additive particles are covered with a thin silicone layer, the water-insoluble hydrophilic additives incorporated into pre-polymers affect the E. coli attachment on the surface of the foam by increased surface roughness; • Water-soluble additives, such as tannic acid, show a considerable antibacterial effect when dissolving from the polymer matrix; • During the 24-h incubation period, the gram-negative bacteria E. coli is more prone to adhere to polysiloxane-based elastomer surface than to polyurethane-based foam. However, the bacterial concentration in the surrounding medium of the pristine polysiloxane is lower than for a standard polyurethane.
We conclude that using low-cost natural additives without dip-coating but initial incorporation into the polymer matrix makes it possible to avoid microbial biofilm formation on the surface of silicone foams. For future research, it would be essential to analyse the variations in filler content in the range where the mechanical properties of the elastomer are acceptable for a desired application.

Data availability
The datasets supporting the conclusions of this article are included within the article and its supplementary files. The additional SEM images used and/or analysed during the current study are available from the corresponding author upon reasonable request.